A Spectrophotometric Procedure, Using Carboxypeptidase A, for the Quantitative Measurement of Ochratoxin A
- 1 January 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 59 (1) , 128-129
- https://doi.org/10.1093/jaoac/59.1.128
Abstract
In the method described, ochratoxin A is cleaved into ochratoxin α (free isocoumarin chromophore) and phenylalanine, using carboxypeptidase. Detection is based on the difference in fluorescence excitation spectra of ochratoxin A (380 nm, maximum) and ochratoxin α (340 nm, maximum). The quantitation of ochratoxin A is based on the loss of fluorescence intensity at 380 nm. The method has been used for the quantitative determination of as little as 4 μg ochratoxin A/kg barley and barley meal but it could be extended to other products.Keywords
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