Identification of a vesicular nucleotide transporter
Top Cited Papers
- 15 April 2008
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 105 (15) , 5683-5686
- https://doi.org/10.1073/pnas.0800141105
Abstract
ATP is a major chemical transmitter in purinergic signal transmission. Before secretion, ATP is stored in secretory vesicles found in purinergic cells. Although the presence of active transport mechanisms for ATP has been postulated for a long time, the proteins responsible for its vesicular accumulation remains unknown. The transporter encoded by the human and mouse SLC17A9 gene, a novel member of an anion transporter family, was predominantly expressed in the brain and adrenal gland. The mouse and bovine counterparts were associated with adrenal chromaffin granules. Proteoliposomes containing purified transporter actively took up ATP, ADP, and GTP by using membrane potential as the driving force. The uptake properties of the reconstituted transporter were similar to that of the ATP uptake by synaptic vesicles and chromaffin granules. Suppression of endogenous SLC17A9 expression in PC12 cells decreased exocytosis of ATP. These findings strongly suggest that SLC17A9 protein is a vesicular nucleotide transporter and should lead to the elucidation of the molecular mechanism of ATP secretion in purinergic signal transmission.Keywords
This publication has 60 references indexed in Scilit:
- Characterization of DIDS‐sensitive ATP accumulation in brain synaptic vesiclesFEBS Letters, 2006
- Validation of Reference Genes for Real-Time Polymerase Chain Reaction Studies in Atlantic SalmonMarine Biotechnology, 2006
- The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalizationAnalytical Biochemistry, 2005
- Real-time RT-PCR normalisation; strategies and considerationsGenes & Immunity, 2005
- Normalization of gene expression measurements in tumor tissues: comparison of 13 endogenous control genesLaboratory Investigation, 2005
- Identification of membrane type-1 matrix metalloproteinase as a target of hypoxia-inducible factor-2α in von Hippel–Lindau renal cell carcinomaOncogene, 2004
- Chipping away at the chip bias: RNA degradation in microarray analysisNature Genetics, 2003
- Validation of endogenous controls for gene expression analysis in microdissected human renal biopsiesKidney International, 2003
- Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assaysJournal of Molecular Endocrinology, 2000
- Amino acid neurotransmission: spotlight on synaptic vesiclesTrends in Neurosciences, 1990