Soybean DRE-binding transcription factors that are responsive to abiotic stresses

20 April 2005

journal article
research article
Published by Springer Nature in Theoretical and Applied Genetics

Vol. 110 (8) , 1355-1362
https://doi.org/10.1007/s00122-004-1867-6

Abstract

Three DREB homologue genes, GmDREBa,GmDREBb, and GmDREBc, were isolated from soybean, Glycine max (L.) Merr. Each of the deduced proteins contains an AP2 domain of 64 amino acids. Yeast one-hybrid assay revealed that all of the three dehydration-responsive, element-binding proteins specifically bound to the dehydration-responsive element. Analysis of transcriptional activation abilities of these proteins in yeast indicated that GmDREBa and GmDREBb could activate the expression of a reporter gene, whereas GmDREBc could not. The transcriptions of GmDREBa and GmDREBb were induced by salt, drought, and cold stresses in leaves of soybean seedlings. The expression of GmDREBc was not significantly affected in leaves but apparently induced in roots by salt, drought, and abscisic acid treatments. These results suggest that these three genes function specifically in response to abiotic stresses in soybean.

Keywords

This publication has 23 references indexed in Scilit:

CBF2/DREB1C is a negative regulator ofCBF1/DREB1BandCBF3/DREB1Aexpression and plays a central role in stress tolerance inArabidopsis
Proceedings of the National Academy of Sciences, 2004
Mutations in the Ca²⁺/H⁺ Transporter CAX1 Increase CBF/DREB1 Expression and the Cold-Acclimation Response in Arabidopsis
Plant Cell, 2003
The calcium sensor CBL1 integrates plant responses to abiotic stresses
The Plant Journal, 2003
ICE1: a regulator of cold-induced transcriptome and freezing tolerance inArabidopsis
Genes & Development, 2003
An EREBP/AP2-type protein in Triticum aestivum was a DRE-binding transcription factor induced by cold, dehydration and ABA stress
Theoretical and Applied Genetics, 2003
DNA-Binding Specificity of the ERF/AP2 Domain of Arabidopsis DREBs, Transcription Factors Involved in Dehydration- and Cold-Inducible Gene Expression
Biochemical and Biophysical Research Communications, 2002
Overexpression of the Tobacco Tsi1 Gene Encoding an EREBP/AP2–Type Transcription Factor Enhances Resistance against Pathogen Attack and Osmotic Stress in Tobacco
Plant Cell, 2001
The Arabidopsis CBF Gene Family Is Composed of Three Genes Encoding AP2 Domain-Containing Proteins Whose Expression Is Regulated by Low Temperature but Not by Abscisic Acid or Dehydration1
Plant Physiology, 1999
Low temperature regulation of theArabidopsisCBF family of AP2 transcriptional activators as an early step in cold‐inducedCORgene expression
The Plant Journal, 1998
Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit
Proceedings of the National Academy of Sciences, 1997