Humanization of mouse anti-human IL-2 receptor antibody B-B10
- 1 January 1994
- journal article
- Published by Oxford University Press (OUP) in Protein Engineering, Design and Selection
- Vol. 7 (3) , 435-443
- https://doi.org/10.1093/protein/7.3.435
Abstract
Mouse monoclonal anti-human IL-2 receptor antibody (B-B10) inhibits IL-2-dependent human T-cell proliferation. It has been used in clinical trials in the transplantation field and promising results are being accumulated. Mouse B-B10 antibody was humanized by grafting all CDRs and some framework amino acid residues onto human antibodies, KAS for VH and PAY for V kappa. Nine humanized B-B10s with differently grafted framework residues were constructed and assessed for their biological activities. One of these humanized B-B10, M5, showed nearly the same activity as the mouse B-B10. The 49th residue of V kappa was demonstrated to play a crucial role in the antigen-antibody interaction by 3-D structure analysis with a computer modeling system.Keywords
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