BIOSYNTHESIS OF PORPHYRINS IN WHEAT LEAVES: I. SUCCINATE:CoA LIGASE (ADP)
- 1 October 1965
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 43 (10) , 1605-1614
- https://doi.org/10.1139/o65-177
Abstract
Succinate:CoA ligase (ADP) (E.C. No. 6.2.1.5) was isolated and partially purified from acetone powders of wheat leaves. The enzyme is specific for succinate and ATP, but is inhibited by concentrations of ATP above 4.5 × 10−3 M and requires either Mg++or Mn++ions. Kmvalues for succinate, ATP, CoA, Mg++, and MH++are 3.2 × 10−2 M, 1.3 × 10−3 M, 9.5 × 10−5 M, 4.1 × 10−3 M, and 3.7 × 10−3 M respectively. The enzyme is localized within chloroplasts and catalyzes a termolecular reaction.This publication has 13 references indexed in Scilit:
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