SEQUENCE OF A CDNA-ENCODING HUMAN GALACTOSE-1-PHOSPHATE URIDYL TRANSFERASE

  • 1 August 1990
    • journal article
    • research article
    • Vol. 7  (4) , 365-369
Abstract
We report a revised sequence of a cDNA that encodes a human galactose-1-phosphate uridyl transferase. The cDNA is 1295 bases in length and encodes a 43,000 Mr protein. The sequence was derived from a cDNA clone isoalted from a transformed human lymphoblast cell line and amplified in a polymerase chain reaction. The revised sequence reveals a high degree of amino acid conservation between the human enzyme and the homologous enzymes from Escherichia coli and yeast than was previously thought to exist.

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