Steroid 21‐hydroxylase is a major autoantigen involved in adult onset autoimmune Addison's disease
- 31 August 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 309 (1) , 51-55
- https://doi.org/10.1016/0014-5793(92)80737-2
Abstract
An adrenal-specific protein reacting with autoantibodies in the sera of patients with adult onset Addison's disease has been purified from human adrenal glands. The protein, mol.wt. 55K, has the biochemical characteristics of steroid 21-hydroxylase and reacts on Western blots with rabbit antibodies to recombinant 21-hydroxylase. Absorption of the native human 55K adrenal protein with human adrenal autoantibodies prevented the subsequent reaction of the 55K protein with rabbit antibodies to 21-hydroxylase in Western blot analysis. In addition, human adrenal autoantibodies reacted with recombinant 21-hydroxylase expressed in yeast. These data indicate that the adrenal specific enzyme steroid 21-hydroxylase is a major autoantigen involved in adult onset autoimmune Addison's disease.Keywords
This publication has 17 references indexed in Scilit:
- Identification by molecular cloning of an autoantigen associated with Addison's disease as steroid 17α-hydroxylaseThe Lancet, 1992
- Expression and Functional Study of Wild-Type and Mutant Human Cytochrome P450c21 inSaccharomyces cerevisiaeDNA and Cell Biology, 1991
- Control of Yeast Mating Signal Transduction by a Mammalian β 2 -adrenergic Receptor and G s α SubunitScience, 1990
- The natural history of adrenal function in autoimmune patients with adrenal autoantibodiesJournal of Endocrinology, 1988
- Immunoprecipitation of human adrenal microsomal antigenFEBS Letters, 1988
- Reaction of monoclonal antibodies with plasma membrane proteins after binding on nitrocellulose: Renaturation of antigenic sites and reduction of nonspecific antibody bindingAnalytical Biochemistry, 1987
- COMPLEMENT-FIXING ADRENAL AUTOANTIBODIES AS A MARKER FOR PREDICTING ONSET OF IDIOPATHIC ADDISON'S DISEASEThe Lancet, 1983
- DEVELOPMENT OF ADRENOCORTICAL FAILURE IN NON-ADDISONIAN PATIENTS WITH ANTIBODIES TO ADRENAL CORTEX A CLINICAL FOLLOW-UP STUDYClinical Endocrinology, 1982
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- AUTO-ANTIBODIES IN ADDISON'S DISEASEThe Lancet, 1957