Purification and Properties of Amine Oxidase from Epicotyls of Pisum sativum
- 1 May 1971
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 47 (5) , 644-648
- https://doi.org/10.1104/pp.47.5.644
Abstract
A procedure has been developed for the purification of amine oxidase (E.C. 1.4.3.4) from etiolated pea epicotyls (Pisum sativum cv. Little Marvel). The enzyme is sensitive to copper chelating reagents and carbonyl reagents, but is not inhibited by sulfhydryl reagents. The purified enzyme has a molecular weight of 1.85 × 105, as determined by sedimentation equilibrium centrifugation, and has been shown to be specifically stimulated by phosphate.Keywords
This publication has 19 references indexed in Scilit:
- Aspects of catalysis by the amine oxidase of pea seedlingsBiochemistry, 1970
- High resolution acrylamide gel electrophoresis of histonesArchives of Biochemistry and Biophysics, 1969
- Amine oxidase. XII. Association and dissociation, and number of subunits of beef plasma amine oxidaseBiochemistry, 1968
- Isolation and Properties of the Enzyme System Forming Indoleacetic AcidPlant Physiology, 1967
- Pyridoxal phosphate as a constituent of the histaminase (benzylamine oxidase) of pig plasmaProceedings of the Royal Society of London. B. Biological Sciences, 1965
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- Benzylamine oxidase and histaminase: purification and crystallization of an enzyme from pig plasmaProceedings of the Royal Society of London. B. Biological Sciences, 1964
- Equilibrium Ultracentrifugation of Dilute Solutions*Biochemistry, 1964
- The nature of the prosthetic groups of plasma amine oxidaseBiochemical and Biophysical Research Communications, 1962
- A Sensitive Method for the Estimation of Hydrogen Peroxide in Biological MaterialsNature, 1955