Radioimmunoassay for human corticosteroid-binding globulin.
- 1 July 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 29 (7) , 1389-1391
- https://doi.org/10.1093/clinchem/29.7.1389
Abstract
We isolated corticosteroid-binding globulin from human plasma and used the isolated protein as the antigen in raising antibodies in rabbits. The resulting antiserum was used to develop an RIA with which we can determine the globulin in as little as 0.007 microL of serum, although we routinely use, after appropriate dilution, 0.02 microL. The mean concentration of corticosteroid-binding globulin in the serum of 40 normal subjects was 32.4 (SD 4.2) mg/L. The correlation (r) between results with this RIA and those by an established binding assay was 0.94.This publication has 3 references indexed in Scilit:
- Steroid-protein interactions. Human corticosteroid-binding globulin: characterization of dimer and electrophoretic variantsBiochemistry, 1982
- ISOLATION, CHARACTERIZATION AND RADIOIMMUNOASSAY OF CORTICOSTEROID-BINDING GLOBULIN (CBG) IN HUMAN SERUM – CLINICAL SIGNIFICANCE AND COMPARISON TO THYROXINE-BINDING GLOBULIN (TBG)Acta Endocrinologica, 1979
- Identification of Corticosteroid-Binding Globulin in Human Milk: Measurement with a Filter Disk AssayJournal of Clinical Endocrinology & Metabolism, 1976