Determination of the Optical Purity for Serine and Threonine by Gas Chromatography on a Chiral Column
- 1 August 1997
- journal article
- Published by Oxford University Press (OUP) in Chemistry Letters
- Vol. 26 (8) , 779-780
- https://doi.org/10.1246/cl.1997.779
Abstract
In a two-step procedure, serine and threonine were converted first to N-isobutoxycarbonyl-amino acid 2,2,2-trifluoroethyl esters and subsequently also to novel N,O-bis-isobutoxycarbonyl-derivatives. As such all four isomers of threonine could easily be resolved on a commercial chiral (Chirasil-d-Val) gas chromatography column at 140 °C. A significant influence of the configuration at the β-position on the retention time was thereby observed. Similarly, improved separation was demonstrated for the two enantiomers of N,O-bis-isobutoxycarbonyl-serine isobutylamide.Keywords
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