DETECTION OF CLOSTRIDIUM-DIFFICILE ON MINIMAL AND SELECTIVE MEDIUM AND BY IMMUNOFLUORESCENCE ANTIBODY STAINING

  • 1 January 1981
    • journal article
    • research article
    • Vol. A132  (2) , 149-157
Abstract
A minimal and selective medium containing 5 amino acids, 3 vitamins, a low sugar concentration and 2 antibiotics (cefoxitin at 16 .mu.g/ml and streptomycin at 500 .mu.g/ml) is described for isolation of C. difficile from the gut. Comparable results were obtained with this medium and using spore isolation by the sodium thioglycolate-lysozyme technique. An antiserum specific to C. difficile was prepared and used for detection by immunofluorescent antibody staining. This sensitive technique led to a quick and early identification of this bacteria.

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