Determination of erythrocyte pyrimidine 5′-nucleotidase activity by31P nuclear magnetic resonance: Comparison of normal controls and multiple sclerosis patients
- 1 September 1986
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 42 (9) , 1016-1018
- https://doi.org/10.1007/bf01940712
Abstract
Summary A technique to assay erythrocyte pyrimidine 5′-nucleotidase activity in situ using31P nuclear magnetic resonance spectroscopy is presented. The assay is chemically specific, simple and applicable to untreated lysates. A comparison of enzyme levels in normal controls and in multiple sclerosis patients employing the assay yielded no significant differences between both groups. Difficulties encountered in the quantitative analysis of the assay using1H-NMR spectroscopy are briefly discussed.Keywords
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