Formation and Verification of the Structure of the 1-Fluorenylmethyl Chloroformate Derivative of Sulfamethazine

Publisher Website
Google Scholar
Abstract
Sulfamethazine (SMZ) is derivatized with 1-fluorenylmethyl chloroformate (FMOC) to form the fluorescent adduct SMZ−FMOC. Conditions for formation are optimized with respect to pH, reagent concentration, and reagent ratio. Reagent and product profiles (including the hydrolysis byproduct FMOC-OH) versus time are followed by reversed phase HPLC with UV absorbance detection. FMOC−SMZ has been crystallized, its composition confirmed by microanalysis, and its structure corroborated by IR and NMR spectroscopy. From 10 down to 1 ppm, there is clear gentle curvature in the fluorescence intensity of SMZ−FMOC. The linear response range extends from above 100 ppb down to about 100 ppt, and an increase in sensitivity for the fluorescent detection of FMOC−SMZ (over the usual UV absorbance detection of SMZ) is calculated to be better than 3 orders of magnitude.

This publication has 0 references indexed in Scilit: