A Comparison of the Methods of X-ray Microanalysis, Compartmental Analysis and Longitudional Ion Profiles to Estimate Cytoplasmic Ion Concentrations in Two Maize Varieties

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Abstract
Hajibagheri, M. A., Flowers, T. J., Collins, J. C. and Yeo, A. R. 1988. A comparison of the methods of X-ray microanalysis, compartmental analysis and longitudinal ion profiles to estimate cytoplasnuc ion concentrations in two maize varieties.—J. exp. Bot. 39: 279-290. The ion content of compartments within plant root cells has been studied by three different methods; flux analysis using radioactive isotopes, longitudinal ion profiles and X-ray microanalysis. The data provide estimates of the concentrations of K+ and CI− in the cytoplasm of roots of culture solution and salt grown maize by three independent methods. In the cultivar LG11 grown in 50 mol m−3 NaCl X-ray microanalysis, compartmental analysis and longitudinal profiles yielded approximately agreeing values for cytoplasmic K+ of 90, 70 and 62 mol m−33 of tissue volume respectively. However, the methods disagreed on cytoplasmic Cl− where the value obtained by compartmental analysis was about four times that from X-ray microanalysis and longitudinal profiles. Possible reasons for this are discussed.