Attenuation of white gene expression in transgenic Drosophila melanogaster: Possible role of a catalytic antisense RNA
- 1 January 1993
- journal article
- research article
- Published by Wiley in Developmental Genetics
- Vol. 14 (4) , 258-265
- https://doi.org/10.1002/dvg.1020140403
Abstract
We have transformed Drosophila melanogaster with a DNA construct encoding a catalytic antisense RNA directed against the primary white gene RNA transcript. Total RNA isolated from transformed flies was shown to catalyze the specific cleavage of in vitro transcribed substrate RNA, indicating the expression of a functional ribozyme. Moreover, transgenic lines carrying homozygous copies of this construct depict a further clear‐cut reduction in eye pigmentation when present in a genetic background that has a priori reduced levels of white gene expression. © 1993Wiley‐Liss, Inc.Keywords
This publication has 39 references indexed in Scilit:
- Ribozymes designed to inhibit transformation of NIH3T3 cells by the activated c-Ha-ras geneGene, 1992
- Induced expression of a Drosophila hsp70 promoter-fusion transgene is reduced after repeated heat shocksGenetics Research, 1992
- Generation of Minute phenotypes by a transformed antisense ribosomal protein geneDevelopmental Genetics, 1992
- Catalytic antisense RNAs produced by incorporating ribozyme cassettes into cDNAGene, 1991
- Molecular genetics of pecanex, a maternal-effect neurogenic locus of Drosophila melanogaster that potentially encodes a large transmembrane proteinDevelopmental Biology, 1989
- Probing Drosophila gene function by antisense RNAGenome, 1989
- Abdominal segmentation of the Drosophila embryo requires a hormone receptor-like protein encoded by the gap gene knirpsNature, 1988
- Vectors for Drosophila P-element-mediated transformation and tissue culture transfectionGene, 1988
- Antisense DNA and RNA: progress and prospects.Genes & Development, 1988
- Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryosNature, 1985