STRUCTURE OF ACRIDINES AND THEIR EFFECT ON RNA-SYNTHESIS INVITRO

  • 1 January 1977
    • journal article
    • research article
    • Vol. 24  (1) , 35-44
Abstract
Ledakrin (C-283), a 1-nitro-9-aminopropylacridine derivative, inhibits RNA synthesis in vitro if the complex with DNA is formed in the presence of thiol. Using several analogues of Ledakrin, it was found that the 1-nitro group is essential for enhancement of the inhibition by thiol; the length of 9-aminoalkyl side chain also plays a role in the reaction between DNA and the dye. The low inhibitory effect of Ledakrin in the absence of thiol compounds appears to be due to a steric hindrance between neighboring 1-nitro and 9-aminoalkyl groups. This hypothesis was confirmed by assaying inhibition of RNA synthesis by several analogues of Ledakrin. [Escherichia coli DNA-dependent RNA polymerase [EC [EC 2.7.7.6] and calf thymus DNA were used in the RNA synthesis in vitro system.].

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