Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene
Open Access
- 1 October 1993
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 175 (19) , 6354-6357
- https://doi.org/10.1128/jb.175.19.6354-6357.1993
Abstract
Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.Keywords
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