Roles of cis-acting elements and chromatin structure inDrosophilaalcohol dehydrogenase gene expression
Open Access
- 12 October 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (19) , 7903-7920
- https://doi.org/10.1093/nar/15.19.7903
Abstract
The alcohol dehydrogensse (Adh) gene of D. melanogaster is transcribed from two different promoters during fly development: the distal (adult) and the proximal (embryonic-larval). Certain aspects of Adh hene regulation are represented in Drosophils continuous cell lines. We have used Drosophils tissue culture cells in an in vivo transient expression essay to delimit cis-acting sequences affecting Adh expression , and to investigate the role of chromatin structure in the distal promoter can exist in at least 2 alternative chromatin configurations. There is a close correlation between specific transcriptional activity of the Adh distal promoter and defined, localized chromatin structural change that indicates altered DNA - Protein inteeractions. Thus, chromatin structure appears to play a role in regulatory sequences of Adh to transcription factors and the transcription machinery.Keywords
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