UTILIZATION OF 2'-DEOXY-6-THIOGUANOSINE 5'-TRIPHOSPHATE IN DNA-SYNTHESIS INVITRO BY DNA POLYMERASE-ALPHA FROM CALF THYMUS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 39  (10) , 3955-3958
Abstract
[Past studies indicated that incorporation of 6-thioguanine into DNA correlates with a carcinostatic response. This compound is converted to its nucleotide forms in living cells.] Chemically synthesized .beta.-2''-deoxy-6-thioGTP, a putative active form of .beta.-2''-deoxy-6-thioguanosine, was used efficiently as a substrate for DNA synthesis catalyzed by DNA polymerase .alpha. from calf thymus. The deoxythioguanylate was incorporated into DNA by replacing deoxyguanylate and supported the further elongation of DNA chains on activated calf thymus DNA. DNA polymerase .beta. used .beta.-2''-deoxy-6-thioGTP at a much lower rate. The reaction product of DNA polymerase .alpha., 6-thioguanine-containing DNA, adsorbed specifically to the organomercurial agarose column and showed a peak of UV absorption at 342 nm, which is characteristic of thioguanine.

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