DNA Ploidy in Testicular Germ Cell Neoplasms Histogenetic and Clinical Implications

Abstract
Flow cytometry was used to determine the DNA ploidy pattern of 148 testicular germ cell neoplasms (seminomas and nonseminomas in pure and mixed histologic phenotypes) and in situ carcinoma (CIS) adjacent to these tumors. The great majority (96.0%) manifested aneuploid DNA contents with minimal intratumoral heterogeneity (2.5%). The mean DNA indices (DI) of CIS (1.7 ± 0.18), pure seminoma (1.82 ± 0.55), and the seminoma component of mixed germ cell neoplasms (1.76 ± 0.13) were statistically similar. The mean DI of nonseminomas pure (1.46 ± 0.29) or as a component of mixed tumors (1.43 ± 0.32) was significantly lower (p

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