Temporal relationship between glucose 6-phosphate dehydrogenase activity and DNA-synthesis
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 50 (3) , 207-215
- https://doi.org/10.1007/bf00491068
Abstract
Wounds have been inflicted in the skin of guinea-pigs. Measurements were made on the basal cells 39–54 h later, at different times of the day. It has been shown that there can be two peaks of mitosis, one about mid-day and the other about 22 h. Synthesis of DNA, measured by Feulgen microdensitometry, preceded mitotic activity. Marked changes were found in glucose 6-phosphate dehydrogenase activity, measured by quantitative cytochemistry and microdensitometry. The greatest activity preceded DNA-synthesis, indicating that pentose-shunt metabolic activity is involved in biosynthetic processes required for cell proliferation.This publication has 4 references indexed in Scilit:
- Regulation of the pentose phosphate cycleBiochemical Journal, 1974
- Studies on the reduction of tetrazolium saltsHistochemistry and Cell Biology, 1973
- Energy metabolism in healing skin woundsJournal of Surgical Research, 1970
- Enzyme activities in the repairing epithelium during wound healingJournal of Surgical Research, 1970