Complete amino acid sequence of human brain calmodulin

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Abstract
The complete amino acid sequence of calmodulin from human brain was determined by using peptides derived from digests with trypsin, Staphylococcus aureus V8 protease and cyanogen bromide. The peptides were purified by reversed-phase high-performance liquid chromatography and analyzed with a sequenator. The protein contains 148 amino acid residues and has a MW of 16,792. As in other calmodulins, the amino-terminal residue of the protein is blocked by an acetyl group, and a trimethyllysine residue is located at position 115. The only difference between this sequence and those fully determined in other species is the assignment of amide groups.