PLOIDY AND PROLIFERATION PATTERNS IN COLORECTAL ADENOCARCINOMAS RELATED TO DUKES CLASSIFICATION AND TO HISTOPATHOLOGICAL DIFFERENTIATION - A FLOW-CYTOMETRIC DNA STUDY

Google Scholar
  • 1 January 1983
    • journal article
    • research article
    • Vol. 91  (2) , 89-95
Abstract
The cellular DNA pattern in 66 [human] colorectal adenocarcinomas was studied by flow-cytometric DNA analysis. The degree of ploidy and the proportion of cells in S-phase were related to the clinical stage according to Dukes'' classification and to the histological differentiation. Multiple cell populations were found in about 60% of the tumors but more frequently in advanced clinical stages. According to the DNA index the cell populations were bimodally distributed with 1 peak in the diploid-peridiploid region and 1 peak in the tri- to tetraploid region. In the 2nd group there was a higher frequency of more advanced tumors compared to the first. The proportion of cells in S-phase was higher in pure diploid tumor cell populations of all clinical stages compared to normal mucosa but lower compared to peridiploid and aneuploid cell populations with high DNA index. High and low S-phase values may occur in all clinical stages, but a significant higher mean value was found for Duke''s C compared to Dukes'' B tumors. Distant metastases occur at all DNA indices and with various S-phase values. Tumors of different clinical stages and histological differentiation may be subdivided according to DNA index, to the existence of single or multiple cell populations and to the proportion of cells in S-phase. The biological significance of this subdivision can only be evaluated by clinical follow-up.

This publication has 1 reference indexed in Scilit: