In vivo Assay of Viability of Amastigotes of Leishmania donovani

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Abstract
Following i.v. injection of formalin-killed or UV-irradiated amastigotes of L. donovani, the parasites could be identified in mouse liver at 5 min, but most amastigotes were digested by 3 h or between 24-72 h, respectively. An in vivo assay of viability, based on these observations, suggests differences in the viability of hamster amastigote populations. The identification of dead and/or dying amastigotes in the liver 24 h after i.v. injection suggests that enumeration of amastigotes from Giemsa-stained slide preparations may include varying numbers of nonviable organisms.

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