Peroxidases from Phaeophyceae II: Analytical Disc-Electrophoresis and Isoelectric Focusing of Peroxidases from Phaeophyceae

Publisher Website
Google Scholar
Abstract
Peroxidase-containing extracts of 6 Fucales [Ascophyllum nodosum, Pelvetia canaliculata, Fucus spiralis, F. serratus, F. vesiculosus, Cystoseira baccata] and 5 Laminariales [Laminaria digitata, L. saccharina, L. hyperborea, L. ochroleuca, Saccorhiza polyschides] were examined. Using analytical disc-electrophoresis in polyacrylamide gels, the Fucales show certain correlations in the pattern of the peroxidases. The Laminariales, however, differ completely from these, and also the various Laminariales have their own unique pattern. With isoelectric focusing with polyacrylamide gels not containing urea, it was not possible to obtain focusing. In the presence of urea, the peroxidases of the Laminariales proved to be unstable; in the case of the Fucales it was possible to identify bands showing peroxidase activity in the region of pH 4.1 to 4.5.