Characterization of plasma membrane redox activity from ehrlich cells
- 1 June 1994
- journal article
- research article
- Published by Wiley in Cell Biochemistry and Function
- Vol. 12 (2) , 149-152
- https://doi.org/10.1002/cbf.290120211
Abstract
Ferricyanide reductase activity of plasma membranes isolated from Ehrlich ascites tumour cells was very sensitive to trypsin treatment. The decreases of activity observed after treatment with different glycosidases suggests that ferricyanide reductase is a glycoprotein. The opposite effects of phospholipase A2 and phospholipase C on the redox activity indicate that the phospholipidic environment plays an important role in the function of ferricyanide reductase. Sodium ions at millimolar concentrations, and some divalent cations at micromolar concentrations (Ca2+, Mg2+, Sr2+, and Mn2+) behaved as stimulators of ferricyanide reductase activity.Keywords
This publication has 19 references indexed in Scilit:
- Sodium-dependentl-serine transport in plasma membrane vesicles isolated from Ehrlich cells by two-phase compartmentationThe Journal of Membrane Biology, 1991
- Transmembrane ferricyanide reductase activity in Ehrlich ascites tumor cellsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1988
- Cell surface glycoconjugates control the activity of the NADH-ascorbate free radical reductase of rat liver plasma membraneBiochemical and Biophysical Research Communications, 1988
- Inositol phosphates turnover, cytosolic Ca++ and pH: Putative signals for the control of cell growthLife Sciences, 1986
- Modulation of cytosolic protein kinase C activity by ferricyanide: priming event seems transmembrane redox signallingFEBS Letters, 1986
- Transplasma-membrane redox systems in growth and developmentBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1985
- Monodehydroascorbate as an electron acceptor for NADH reduction by coated vesicle and Golgi apparatus fractions of rat liverBiochimica et Biophysica Acta (BBA) - General Subjects, 1984
- The involvement of the membrane oxidoreduction system in stimulating amino acid uptake in ehrlich ascites tumor cellsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1981
- Isolation and partial characterization of human erythrocyte membrane NADH: (Acceptor) oxidoreductaseJournal of Supramolecular Structure, 1978
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976