Effect of culture density on cyclic-AMP responses of rabbit aortic smooth-muscle cells to hormonal stimulation

1 August 1981

journal article
research article
Published by Portland Press Ltd. in Bioscience Reports

Vol. 1 (8) , 643-649
https://doi.org/10.1007/bf01116280

Abstract

Low-density cultures of smooth-muscle cells from explants of rabbit thoracic aorta responded to isoprenaline and prostaglandin [PG] E1 and PGI2 with marked rises in cAMP levels. High-density cultures were completely unresponsive to isoprenaline and showed diminished responsiveness to PG. Low-density cultures were very rapidly desensitized by isoprenaline (t1/2 [half-life] .apprx. 15 min); desensitization by PGE1 was much slower (t1/2 .apprx. 95 min).

Keywords

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