Phosphatase Activity in Plant Cells with Special Reference to Those Treated with Colchicine and Gammexane

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Abstract
A study was made of the differential nature of the chromosomes and other cell components on the basis of their phosphatase activity. Differential precipitation was very high for all cellular components for alkaline phosphatase, but low and uniform for acid phosphatase. The presence of the enzyme in both nucleic acid and protein of the chromosomes accounts for the high alkaline phosphatase activity of these structures. The end portion of the chromosomes have a high enzyme content, but it is low in the constriction regions. There is decreased phosphatase activity in polyploid cells because of the greater dispersion of the enzyme as a result of the increase in cell surface. It is suggested that the loss of distinction between nucleoli in polyploid cells is caused by lack of viscosity differential between nucleoli and nucleoplasm.

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