Structural and functional characterization of full-length heparin-binding growth associated molecule.
Open Access
- 1 January 1992
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 3 (1) , 85-93
- https://doi.org/10.1091/mbc.3.1.85
Abstract
Heparin-binding growth-associated molecule (HB-GAM) was purified from adult bovine brain and chicken heart. The yield of HB-GAM is increased by 5- to 10-fold when 250 mM NaCl is added to the homogenization buffer, indicating that HB-GAM may exist as a complex with an insoluble component of the tissue. The complete amino acid sequence of the brain-derived HB-GAM was established by automated Edman degradation of the intact protein and chemically or enzymatically derived fragments. The mass of bovine HB-GAM as determined by plasma desorption time-of-flight mass spectrometry is 15,291 mass units, which compares favorably with the calculated mass of 15,289 based on the amino acid sequence. Therefore, HB-GAM has not undergone any major post-translational modifications other than cleavage of the signal peptide. These results indicate that previous amino acid sequence analysis of this protein was carried out using truncated HB-GAM. Full-length HB-GAM is not a mitogen for Balb/3T3 clone A31, Balb MK, NRK, or human umbilical vein endothelial cells. HB-GAM does, however, have adhesive properties and neurite extension activity for chick embryo cerebral cortical derived neurons when presented to these cells as a substrate. HB-GAM had little neurite extension activity when presented as a soluble factor.Keywords
This publication has 21 references indexed in Scilit:
- Molecular cloning of RI-HB, a heparin binding protein regulated by retinoic acidBiochemical and Biophysical Research Communications, 1991
- Possible dissociation of the heparin-binding and mitogenic activities of heparin-binding (acidic fibroblast) growth factor-1 from its receptor-binding activities by site-directed mutagenesis of a single lysine residue.The Journal of cell biology, 1990
- Heparin-binding neurotrophic factor (HBNF) and MK, members of a new family of homologous, developmentally regulated proteinsBiochemical and Biophysical Research Communications, 1990
- A retinoic acid responsive gene, MK, produces a secreted protein with heparin binding activityBiochemical and Biophysical Research Communications, 1990
- A novel 17 kD heparin-binding growth factor (HBGF-8) in bovine uterus: Purification and N-terminal amino acid sequenceBiochemical and Biophysical Research Communications, 1989
- Identification of a new heparin‐binding protein localized within chick basement membranesEuropean Journal of Biochemistry, 1989
- cDNA cloning and sequencing of a new gene intensely expressed in early differentiation stages of embryonal carcinoma cells and in mid-gestation period of mouse embryogenesisBiochemical and Biophysical Research Communications, 1988
- Molecular Weight Determinations of Proteins by Californium Plasma Desorption Mass SpectrometryScience, 1984
- BALB and kirsten murine sarcoma viruses alter growth and differentiation of EGF-dependent BALB/c mouse epidermal keratinocyte linesCell, 1983
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970