The origin and properties of β-lactamase satellite bands seen in isoelectric focusing
- 1 August 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 12 (2) , 127-131
- https://doi.org/10.1093/jac/12.2.127
Abstract
Citrobacter diversus 2046E and Branhamella catarrhalis 2001 E each produce a constitutive, chromosomally mediated broad-spectrum β-lactamase. Isoelectric focusing of both enzymes revealed patterns of multiple ‘satellite’ bands. The principal satellite bands of each enzyme were isolated and characterized. Individual bands of each enzyme gave similar substrate profiles, molecular weights and responses to inhibitors to one another. The results support the theory that satellite bands are due to the loss and/or modification of specific amino acid residues resulting in biologically active molecules with altered nett charges.Keywords
This publication has 1 reference indexed in Scilit:
- Identification of -Lactamases by Analytical Isoelectric Focusing: Correlation with Bacterial TaxonomyJournal of General Microbiology, 1976