Pr-1, a novel antifungal protein from pumpkin rinds
- 17 September 2009
- journal article
- Published by Springer Nature in Biotechnology Letters
- Vol. 32 (1) , 125-130
- https://doi.org/10.1007/s10529-009-0126-y
Abstract
A novel antifungal protein, Mr = ca. 40 kDa, was isolated from pumpkin rind and designated Pr-1. When purified by anion exchange chromatography and HPLC, it inhibited growth of several fungi including Botrytis cinerea, Fusarium oxysporum, Fusarium solani and Rhizoctonia solani, as well as the yeast, Candida albicans, at 10–20 μM. It did not inhibit growth of Escherichia coli or Staphylococcus aureus even at 200 μM. Laser scanning microscopy of fungal cells exposed to rhodamine-labeled Pr-1 revealed that the protein accumulated and was localized on the cell surface. Uptake of the vital stain, SYTOX Green, was enhanced when fungal conidia were treated with Pr-1 suggesting that the protein has membrane permeabilization activity. Pr-1 was thermostable at 70°C and did not lyse human red blood cells at 128 μM suggesting that the protein may be useful as an antifungal agent with little, if any human cytotoxicity.Keywords
This publication has 18 references indexed in Scilit:
- Purification and Characterization of an Antifungal Protein, C-FKBP, from Chinese CabbageJournal of Agricultural and Food Chemistry, 2007
- Molecular and functional characterization of a cyclophilin with antifungal activity from Chinese cabbageBiochemical and Biophysical Research Communications, 2007
- Structure‐activity relationship of HP (2–20) analog peptide: Enhanced antimicrobial activity by N‐terminal random coil region deletionPeptide Science, 2007
- Purification and characterization of a heat-stable serine protease inhibitor from the tubers of new potato variety “Golden Valley”Biochemical and Biophysical Research Communications, 2006
- Ribosome-inactivating proteins in edible plants and purification and characterization of a new ribosome-inactivating protein from Cucurbita moschataBiochimica et Biophysica Acta (BBA) - General Subjects, 2006
- Purification and characterization of a novel peptide with antifungal activity from Bothrops jararaca venomToxicon, 2005
- Antifungal proteins and peptides of leguminous and non-leguminous originsPeptides, 2004
- Cloning, Expression, and Characterization of an Antifungal Chitinase fromLeucaena leucocephalade WitBioscience, Biotechnology, and Biochemistry, 2003
- Hispin, a novel ribosome inactivating protein with antifungal activity from hairy melon seedsProtein Expression and Purification, 2002
- Antimicrobial proteins in induced plant defenseCurrent Opinion in Immunology, 1998