Two Nutritional Variants of Cultured Jensen Sarcoma Cells.
- 1 April 1959
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 100 (4) , 862-865
- https://doi.org/10.3181/00379727-100-24805
Abstract
Two nutritional variants (JA-1, JA-2) have been developed from Jensen sarcoma cells cultured in vitro. Although the parental Jensen sarcoma requires asparagine for growth, either strain grows readily in the absence of exogenous asparagine. This variation in nutritional requirement has been retained in JA-1 and JA-2 for 23 and 16 subculture generations in vitro and in 3 and 18 in vivo transplant generations, respectively. This change is stable and heritable under the conditions described. Some growth and histological characteristics of these variants are described.Keywords
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