Heterogeneity ofβ2-Microglobulin in Human Urine
- 1 August 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 43 (8) , 1707-1710
- https://doi.org/10.1080/00021369.1979.10863689
Abstract
Purification and characterization of β2-microglobulin from human urine was performed. The yield was 30.1%, and 150.4 mg of β2-microglobulin was obtained. The final preparation of β2-microglobulin obtained showed three bands on disc gel electrophoresis at pH 9.5, and all of them have immunological activity. However, these three bands migrated as a single band on disc gel electrophoresis at pH 4.3. It is concluded that the three bands observed on disc gel electrophoresis at pH 9.5 were charge isomers. The isoelectric points of isomers were determined by isotachophoresis and two of them were 5.4 and 5.9 respectively, while the other one was not determined.This publication has 5 references indexed in Scilit:
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