Effects of AMPPNP and vanadate on the mechanochemical crossbridge cycle in flagella
- 1 March 1981
- journal article
- research article
- Published by Springer Nature in Journal of Muscle Research and Cell Motility
- Vol. 2 (1) , 131-140
- https://doi.org/10.1007/bf00712064
Abstract
The non-hydrolysable ATP analogue, β,γ-imido-adenosine-5′-triphosphate (AMPPNP, adenylylimidodiphosphate) does not reduce the stiffness of demembranated sea urchin sperm flagella measured in the rigor state in the absence of MgATP2− and does not cause relaxation of rigor bends. The competitive inhibition of flagellar beat frequency by AMPPNP is not enhanced by vanadate. These observations provide further evidence that the dynein-tubulin crossbridge cycle in flagella is similar to the actin-myosin crossbridge cycle.Keywords
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