Characterization of HNK‐1 bearing glycoproteins in human peripheral nerve myelin

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Abstract
The HNK-1 carbohydrate epitope, which is shared by several members of the immunoglobulin gene superfamily, is also the target epitope for IgM anti-MAG autoantibodies in patients with demyelinating neuropathy. By Western blot analysis, there are 7 HNK-1 immunoreactive glycoproteins in human peripheral nerve myelin, two of which have previously been identified as the myelin associated glycoprotein (MAG) and the Po glycoprotein. In this study, the remaining HNK-1 bearing glycoprotein bands were characterized by immunoblot and NH2-terminal sequence analysis, and were all identified as degradation products or aggregates of the Po glycoprotein. MAG and Po are therefore the only HNK-1 bearing glycoproteins in human peripheral nerve myelin.