Electrophoretic Comparison of the Malic Enzyme in Japanese Mosquito (Diptera: Culicidae) Larvae
- 1 May 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Medical Entomology
- Vol. 24 (3) , 376-382
- https://doi.org/10.1093/jmedent/24.3.376
Abstract
Interspecific and intergeneric comparisons of malic enzyme (ME) banding patterns of Japanese mosquito larvae were carried out by horizontal electrophoresis in 5% polyacrylamide gel. Among a total of 56 species and subspecies belonging to 10 genera and 18 subgenera examined, narrow single-banded and monomorphic patterns were commonly observed; in some species two- or three-banded patterns also were detected. Most species showed similar ME band mobilities of Rf 42 to 47. An exceptionally fast-moving band was observed in Anopheles lindesayi japonicus Yamada (Rf 63). All Culex larvae examined possessed a common enzyme activity band with Rf 45. Orthopodomyia anopheloides (Giles) had the slowest band (Rf 33), followed by Malaya genurostris Leicester (Rf 34) and Topomyia yanbarensis Miyagi (Rf 38). Removal of guts from a larva and homogenization in 2-mercaptoethanol intensified ME activity bands in many species.Keywords
This publication has 1 reference indexed in Scilit:
- Identification by Electrophoresis of Culex Adults (Diptera: Culicidae) in Light-Trap Samples1Journal of Medical Entomology, 1984