Cyclic AMP in skin: effects of acute ischaemia

Abstract
The cyclic adenosine 3′,5′-monophosphate (cyclic AMP) content of pig skin was measured several seconds to minutes after removal of the skin from the body. It increased very rapidly, reached a maximum by 2 min after removal (4 times higher than the initial level), then decreased very slowly. Propranolol injected into the animal before or added after the removal of the skin did not suppress this phenomenon. The practical significance of this finding (increase of cyclic AMP level in skin after ischaemia) is obvious—in order to measure cyclic AMP level in vivo, the sample must be frozen immediately to avoid an ‘artificial’ increase in cyclic AMP.