Presence of Anti-Insulin Reaginic Auto-Antibodies of the lgG4 Class in Insulin-Dependent (Type I) Diabetic Patients before Insulin Therapy

Abstract
The autoimmune aetiology of type I diabetes has been well documented. We studied whether anti-insulin anaphylactic antibodies were present on the membrane of basophils from type I diabetics by the toluidine blue method (detecting basophil activation after stimulation by insulin). We observed that basophils of recently diagnosed insulin-dependent diabetic patients (n = 13) were statistically more frequently activated by insulin than basophils from nonin-sulin-dependent diabetics (p

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