The complete sequence and coding content of snowshoe hare bunyavirus small (S) viral RNA species
Open Access
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (12) , 3703-3713
- https://doi.org/10.1093/nar/10.12.3703
Abstract
The complete sequence of the snail (S) viral RNA species of snowshoe hare (SSH) bunyavirus has been determined, principally fran a DNA copy of the RNA cloned in the E.coli plasmid pBr322. The viral S FNA (negative sense strand) is 982 nucleotides long (3.3 × 105 daltons) with conplernentary 5′ and 3′ end sequences. It has a base composition of 30.5%U, 25.8%A, 24.9%C and 18.7%G. In the viral complementary (plus sense) strand there are two overlapping open reading frames initiated by methionine codons. One reading Htdme codes for a 26.8 × 103 dalton protein, the other for a 10.5 × 103 dalton protein. The larger gene product is presumably related to the viral nucleoprotein (N) that is coded by the S Hffi (Gentsch and Bishop (1978) J.Virol. 28, 417–419). The smaller gene product is probably related to the recently identified S RNA coded nonstructural protein (NSS) induced in virus infected cells (Fuller and Bishop (1982) J.Virol. 41, 643–648).Keywords
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