A Type 3 Attenuated Poliovirus Genetically Stable after Human Intestinal Passage.

Abstract
Development of a new type 3 attenuated poliovirus (WM-III) is described. Starting with the W-Fox strain, passage and clone selection at 23°C were employed to develop a strain with improved stability after human intestinal passage. The end result was a virus which had no capacity to grow at 40°C and was not virulent for monkeys injected intracerebrally. The results of tests of fecal virus strains isolated from infants fed the WM-III strain did not show the instability in relation to laboratory markers that has been reported with other attenuated type 3 viruses.

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