Interactions between β2-syntrophin and a family of microtubule-associated serine/threonine kinases
- 1 July 1999
- journal article
- Published by Springer Nature in Nature Neuroscience
- Vol. 2 (7) , 611-617
- https://doi.org/10.1038/10165
Abstract
A screen for proteins that interact with beta 2-syntrophin led to the isolation of MAST205 (microtubule-associated serine/threonine kinase-205 kD) and a newly identified homologue, SAST (syntrophin-associated serine/threonine kinase). Binding studies showed that beta 2-syntrophin and MAST205/SAST associated via a PDZ-PDZ domain interaction. MAST205 colocalized with beta 2-syntrophin and utrophin at neuromuscular junctions. SAST colocalized with syntrophin in cerebral vasculature, spermatic acrosomes and neuronal processes. SAST and syntrophin were highly associated with purified microtubules and microtubule-associated proteins, whereas utrophin and dystrophin were only partially associated with microtubules. Our data suggest that MAST205 and SAST link the dystrophin/utrophin network with microtubule filaments via the syntrophins.Keywords
This publication has 46 references indexed in Scilit:
- Recognition of Unique Carboxyl-Terminal Motifs by Distinct PDZ DomainsScience, 1997
- Increased Activity Associated with the MAST205 Protein Kinase Complex during Mammalian Spermiogenesis1Biology of Reproduction, 1996
- Crystal structure of a PDZ domainNature, 1996
- Expression of Caveolin-3 in Skeletal, Cardiac, and Smooth Muscle CellsJournal of Biological Chemistry, 1996
- β‐Dystroglycan: Subcellular Localisation in Rat Brain and Detection of a Novel Immunologically Related, Postsynaptic Density‐Enriched ProteinJournal of Neurochemistry, 1996
- Dystrophin-related protein is found in the central nervous system of mice at various developmental stages, especially at the postsynaptic membraneJournal of Neuroscience Research, 1994
- The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit.Genes & Development, 1993
- Compartmentalization of cold-stable and acetylated microtubules in the subsynaptic domain of chick skeletal muscle fibreNature, 1990
- Culture conditions affect the cholinergic development of an isolated subpopulation of chick mesencephalic neural crest cellsDevelopmental Biology, 1989
- A rapid procedure for preparing fluorescein-labeled specific antibodies from whole antiserum: its use in analyzing cytoskeletal architecture.The Journal of cell biology, 1983