Rise of Coenzyme A‐Glutathione Mixed Disulfide during Hydroperoxide Metabolism in Perfused Rat Liver

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Abstract
The hepatic metabolism of an externally added hydroperoxide, t‐butyl hydroperoxide, results in substantial decreases in CoASH and acetyl‐CoA and a concomitant increase in CoA‐disulfides. The principal CoA‐disulfide which is formed under these conditions was identified as CoASSG, the mixed disulfide of coenzyme A and glutathione; CoASSCoA levels were unchanged. The perturbation in the coenzyme A system was absent when the hydroperoxide was infused into livers from rats maintained on a selenium‐deficient diet. In these livers, Se‐dependent glutathione peroxidase is lowered to less than 5% of control levels; the observed effects can be attributed to flux through Se‐dependent GSH peroxidase.