ISOLATION OF SPECIFIC ANTIGEN-REACTIVE T-CELL CLONES FROM NUDE (NU-NU) MICE INFECTED WITH MESOCESTOIDES-CORTI

Google Scholar
  • 1 January 1985
    • journal article
    • research article
    • Vol. 54  (2) , 275-279
Abstract
Infection of nude mice with M. corti results in spleen enlargement, with a decrease in percentage positive, but overall increase in Thy-1 positive cells. A low frequency of both parasite antigen-specific and alloantigen-reactive T-cell clones were isolated from the spleen, mesenteric lymph nodes, peritoneal exudate and Peyer''s patches. The T-cell clones from spleen expressed the antigens Thy-1, Lyt-1 and Lyt-2; after stimulation with antigen in the absence of exogenous T cells growth factor (interleukin; IL-2), they produced normal levels of hemopoietic growth factor (IL-3), bone marrow proliferation activity. A higher proportion produced IL-2 compared to clones from normal mice. The isolation of antigen-specific T-cell clones showing normal T-cell characteristics from young nude mice adds weight to suggestions that exposure to antigen results in significant extra-thymic T-cell maturation.