A Zn(II)-translocating P-type ATPase from Proteus mirabilis

1 October 1998

journal article
Published by Canadian Science Publishing in Biochemistry and Cell Biology

Vol. 76 (5) , 787-790
https://doi.org/10.1139/o98-071

Abstract

A mutant of Proteus mirabilis had been previously isolated as defective in swarming. The mutation had been found to be in a gene related to the Escherichia coli zntA gene, which encodes the ZntA Zn(II)-translocating P-type ATPase. In this study the P. mirabilis genewas expressed in an E. coli strain in which the zntA gene had been disrupted. The P. mirabilis gene complemented the sensitivity to salts of zinc and cadmium. Everted membrane vesicles from the zntA-disrupted strain lost ATP-driven ⁶⁵Zn(II) uptake. Membranes from the complemented strain had restored ⁶⁵Zn(II) transport. These results demonstrate that the P. mirabilis homologue of ZntA is a Zn(II)-translocating P-type ATPase.Key words: zinc resistance, P-type ATPase, Proteus mirabilis.

Keywords

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