Glucose 6-Phosphate Formation by L-Type Pentose Phosphate Pathway Reactions of Rat Liver in vitro: Further Evidence

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Abstract
An investigation of the mechanism in vitro of the non-oxidative segment of the pentose phosphate pathway using [5-14C]ribose 5-phosphate as a prediction labeling substrate with rat liver enzyme preparation is reported. G-6--P formed during the initial 0.5 h of reaction was heavily labeled in C-1 and this is consistent with the prediction of the liver (L)-type pentose phosphate pathway (theoretically C-1/C-6 = 0.5). The reaction sequences of the rat (F)-type pentose phosphate pathway exclusively confine 14C to C-6 of G-6-P. The presence of L-type reactions was further affirmed by the formation of D-arabinose 5-phosphate and D-glycero-D-ido-octulose phosphate which were identified and measured during the initial 2 h of incubation using GLC. After 3 h the concentration of 14C in C-1 of G-6-P gradually declined and by 17 h of incubation the ratio of 14C in C-1/C-6 was 0.1. Possible reasons for the late changes in 14C-isotope distributions towards a pattern consistent with a contribution of F-type pentose phosphate pathway are given.