Reversible acetaldehyde inhibition of A23187-stimulated amylase secretion from isolated rat pancreatic acini

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Abstract
The Ca2+ ionophore, A23187, stimulated amylase secretion from isolated rat pancreatic acini in a dosedependent manner with a maximal effect at 6 μM. Acetaldehyde, a metabolite of ethanol, caused a reduction in the magnitude of ionophore-stimulated secretion with no evidence of competitive inhibition. Furthermore, 6 μM ionophore-stimulated amylase secretion was dose-dependently inhibited by acetaldehyde. This inhibitory effect of acetaldehyde, however, was reversible on washing and reincubating acetaldehyde-treated acini. These results suggest that acetaldehyde reversibly inhibits intracellular components mediating stimulated secretion and this inhibition requires a continuous chemical interaction between acetaldehyde and intracellular component(s) regulating stimulated enzyme secretion