New method for detection of Candida albicans in human blood by polymerase chain reaction
- 1 December 1993
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 31 (12) , 3344-7
- https://doi.org/10.1128/jcm.31.12.3344-3347.1993
Abstract
We developed a new method for detection of Candida albicans cells in human blood by the polymerase chain reaction that amplifies a 125-bp region within a species-specific, multiple-copy DNA fragment. In preparing template DNA from C. albicans-containing blood, the addition of C. tropicalis cells, together with antibody which reacts with both cells, raised the sensitivity of detection of C. albicans to about 3 cells per 0.1 ml.Keywords
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