Detection of human coronavirus 229E in nasal washings using RNA:RNA hybridization
Open Access
- 1 September 1989
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 29 (1) , 70-73
- https://doi.org/10.1002/jmv.1890290113
Abstract
A method is described for the detection of human coronavirus 229E (HCV 229E) in nasal washings using RNA:RNA filter hybridization. Volunteers were inoculated with HCV 229E, and daily nasal washings were collected. These washings were then examined for the presence of viral RNA using a single-stranded RNA probe. Nucleic acid hybridization is shown to be a sensitive technique for the diagnosis of HCV 229E infections.Keywords
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