Detection of Human Reagins by Schultz-Dale Technique Using Human Appendix

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Abstract
A study was made to determine the sensitivity of the human appendix in the Schultz-Dale test. An atopic serum, having a titer of 1: 320 by the Prausnitz-Kilstner test, was diluted with Tyrode solution in the ratio of 1:4, 1: 50 and 1:100. Strips of human appendix were passively sensitized with the serum dilutions and separately challenged with 1 mg WSR (water soluble ragweed). Contractions were obtained with all of the serum dilutions. Furthermore, the size of the contractions (relative to the histamine-produced standards) decreased proportionately with increasing serum dilution. Studies in progress show a general correlation between the extent of the Schultz-Dale reaction of the appendix and the Prausnitz-Kustner titers obtained for a series of ragweed-allergic sera. These findings deominstrate for the first time that the human appendix may be employed for the assay of human reagins by the Schultz-Dale technique.

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