Stoichiometry and stability of caldesmon in native thin filaments from sheep aorta smooth muscle

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Abstract
Ca2+-regulated native thin filaments were extracted from sheep aorta smooth muscle. The caldesmon content determined by quantitative gel electrophoresis was 0.06 caldesmon molecule/actin monomer (1 caldesmon molecule per 16.3 actin monomers). Dissociation of caldesmon and tropomyosin from the thin filament and the depolymerization of actin was measured by sedimenting diluted thin filaments. Actin critical concentration was 0.05 .mu.M at I0.1 and 0.13 at I0.05 compared with 0.5 .mu.M for pure F-actin. Tropomyosin was tightly bound, with half-maximal dissociation at less than 0.3 .mu.M thin filaments (actin monomer) under all conditions. Caldesmon dissociation was independent of tropomyosin and not co-operative. The concentration of thin filaments where 50% of the caldesmon was dissociated (CD50) ranged from 0.2 .mu.M (actin monomer) at I0.03 to 8 .mu.M at I0.16 in a 5 mM-MgCl2, pH 7.1, buffer. Mg2+, 25 mM at constant I, increased CD50 4-fold. CD50 was 4-fold greater at 10-4 M-Ca2+ than at 10-9 M-Ca2+. Aorta heavy meromyosin (HMM) .cntdot. ADP .cntdot. Pi complex (2.5 .mu.M excess over thin filaments) strongly antagonized caldesmon dissociation, but skeletal-muscle HMM .cntdot. ADP .cntdot. Pi did not. The behaviour of caldesmon in native thin filaments was indistinguishable from caldesmon in reconstituted synthetic thin filaments. The variability of Ca2+-sensitivity with conditions observed in thin filament preparations was shown to be related to dissociation of regulatory caldesmon from the thin filament.